Extended Life of Human Corneal Endothelial Cells
نویسندگان
چکیده
Methods. Human corneal endothelial cells were transfected with the SV40 large T antigen-coding plasmid pSV3neo using the electroporation method. Transfected and control cells were propagated in culture until senescence. Polymerase chain reaction and immunofluorescence were used to demonstrate messenger RNA and protein, respectively, for the Simian virus 40 large T antigen in the transfected cells. Polymerase chain reaction and hot blotting were used to demonstrate messenger RNA coding for several growth factors and receptors in transfected and control cells.
منابع مشابه
Construction of human corneal endothelial cDNA library and identification of novel active genes.
PURPOSE To describe genes expressed in human corneal endothelial cells and identify novel genes. METHODS Sixteen human donor corneas that had no history of corneal disease, infection, or intraocular surgery were used within 7 days of death. Total RNA was extracted from corneal endothelial cells with attached Descemet membranes. A 3'-directed cDNA library was constructed from mRNA by using a p...
متن کاملExtended incubation times improve corneal endothelial cell transplantation success.
To investigate the ability of extended incubation times to improve the success of endothelial cell transplantation, eight human donor corneas were denuded of their native endothelium, seeded twice during a 1-hr interval with a suspension of cultured infant human corneal endothelial cells, and then incubated for 144 hr under standard conditions. Subsequently the corneas were transplanted into Af...
متن کاملEffect of Fas -670 A/G Gene Polymorphism on Corneal Allograft Endothelial Rejection
Background: Human cornea expresses functional Fas-ligand capable of killing Fas+ activated lymphocytes. Fas expression is partly regulated by -670 A/G polymorphism in the promoter region of Fas gene. Objective: The aim of the present study is to determine the association between Fas-670A/G polymorphism and survival of corneal transplantation. Methods: In 276 graft recipients who mainly underwen...
متن کاملبهکار گیری مدل های طولی در بررسی تاثیر یاگ لیزر ایریدوتومی محیطی بر روی سلول های اندوتلیوم قرنیه در بیماران مشکوک به گلوکوم زاویه بسته اولیه
Introduction: Primary angle closure glaucoma suspects is one of the common causes of blindness in the world and methods of treatment are associated with side effects such as loss of corneal endothelial cells. The purpose of this study was to investigate the effects YAG laser peripheral iridotomy on count and morphology of endothelial cells in patients with primary angle closure glaucoma suspect...
متن کاملEffect of Purification of Human Adipose-derived Mesenchymal Stem Cells on the Expression of vWF Cell Factor Under Chemical and Mechanical Conditions
Introduction: Human adipose-derived mesenchymal stem cells (hADSCs) are easily accessible in the body, and under appropriate conditions, they can be directed toward various phenotypes. Therefore, hADSCs have been considered as a potential cell source for tissue engineering applications. hADSCs are able to differentiate into endothelial cells which covers the interior surface of vessels, in vi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2005